王丰青,博士,教授,博士生导师。中国中西医结合学会分子生药学专业委员会委员,中华中医药学会中药资源学分会委员,中国中药协会中药生态农
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业专业委员会委员。
研究领域:药用植物资源可持续利用;中药材育种;分子生药学
所授课程:药用植物育种学、药用植物育种理论与技术
E-mail: heauzycxw@126.com
教育与研究/工作经历
2022.02-至今 河南农业大学,教授
2015.11-2022.01 河南农业大学,副教授
2011.08-2015.10 河南农业大学,讲师
2008.09-2011.06 浙江大学,作物学,博士学位
承担项目与课题
1. 国家自然科学基金面上项目: 栽培地黄驯化驱动下表型演变与品质形成的分子机制研究(82373985), 2024.1-2027.12, 正研, 主持.
2. 中央本级重大增减支项目子课题: 基于基因编辑技术创制地黄和天目地黄“优形优质”种质材料(2060302), 2022.7-2025.12, 正研, 主持.
3. 国家自然科学基金面上项目: WRKY转录因子调控地黄苯乙醇苷生物合成的机制研究(81872950), 2019.1-2022.12, 结题, 主持.
4. 国家自然科学基金面上项目: 地黄毛蕊花糖苷生物合成的分子调控机制研究(81473299), 2015.1-2018.12, 结题, 主持.
5. 河南省自然科学基金面上项目: 基于系统生物学方法的地黄梓醇生物合成途径研究(162300410157), 2017.1-2018.12, 结题 主持.
6. 中国博士后科学基金项目(二等): 中介体亚基调控地黄连作的分子功能研究(2013M541977), 2013.7-2015.7, 结题, 主持.
7. 河南省博士后科研项目(三等): 响应地黄连作的中介体亚基基因功能研究(2012050), 2013.7-2015.7, 结题, 主持.
8. 河南省教育厅重点项目:地黄远缘杂交技术平台构建及应用研究(22A360009), 2022.1-2023.12, 正研, 主持.
9. 河南省教育厅重点项目: 地黄种质资源收集、评价及耐连作种质筛选(14A360007), 2014.1-2015.12, 结题, 主持.
10. 河南省教育厅重点项目: 怀牛膝种质资源整理与新品种选育(15B360002), 2015.1-2016.12, 结题, 主持.
论文、论著和专利
论文
1. Xin Zuo, Chunyan Miao, Mingming Li, Li Gu, Xu Yang, Ci Song, Mingjie Li, Jiafang Du, Caixia Xie, Xiangyang Liu, Hongzheng Sun, Lianzhen Li, Zhongyi Zhang, and Fengqing Wang (corresponding author). Purple
Rehmannia : investigation of the activation of R2R3-MYB transcription factors involved in anthocyanin biosynthesis. Physiologia Plantarum, 2023, 175(3): e13920.
2. Fengqing Wang, Xinrong Li, Xin Zuo, Mingming Li, Chunyan Miao, Jingyu Zhi, Yajing Li, Xu Yang, Xiangyang Liu, Caixia Xie. Transcriptome-wide identification of WRKY transcription factor and functional characterization of
RgWRKY37 involved in acteoside biosynthesis in
Rehmannia glutinosa. Frontiers in Plant Science, 2021, 12: 739853.
3. Xinrong Li, Xin Zuo, Mingming Li, Xu Yang, Jingyu Zhi, Hongzheng Sun, Caixia Xie, Zhongyi Zhang, Fengqing Wang (corresponding author). Efficient CRISPR/Cas9-mediated genome editing in
Rehmannia glutinosa. Plant Cell Reports, 2021, 40(9), 1695-1707.
4. Xin Zuo, Fengqing Wang (corresponding author), Xinrong Li, Mingming Li. Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in
Rehmannia chingii and
R. henryi. Biologia Plantarum, 2020, 64: 798-806.
5. Jingyu Zhi#, Yajing Li#, Zhongyi Zhang, Chaofei Yang, Xiaotong Geng, Miao Zhang, Xinrong Li, Xin Zuo, Mingjie Li, Yong Huang, Fengqing Wang (corresponding author), Caixia Xie. Molecular regulation of catalpol and acteoside accumulation in radial striation and non-radial striation of
Rehmannia glutinosa tuberous root. International journal of molecular science, 2018, 19(12): 3751.
6. Fengqing Wang, Jingyu Zhi, Zhongyi Zhang, Lina Wang, Yanfei Suo, Caixia Xie, Mingjie Li, Bao Zhang, Jiafang Du, Li Gu, Hongzheng Sun. Transcriptome analysis of salicylic acid treatment in
Rehmannia glutinosa hairy roots using RNA-seq technique for identification of genes involved in acteoside biosynthesis. Frontiers in Plant Science, 2017, 8: 787.
7. Fengqing Wang, Yunhe Tian, He Wei, Yanfei Suo, Caixia Xie, Mingjie Li, Yong Huang, Xinjian Chen, Zhongyi Zhang. Identification and expression analysis of
Rehmannia glutinosa Mediator genes in response to continuous cropping. Acta Physiologiae Plantarum, 2015, 37(12): 264.
8. Fengqing Wang, Yanfei Sou, He Wei, Mingjie Li, Caixia Xie, Lina Wang, Xinjian Chen, Zhongyi Zhang. Identification and characterization of 40 isolated
Rehmannia glutinosa MYB family genes and their expression profiles in response to shading and continuous cropping. International journal of molecular science, 2015, 16(7), 15009-15030.
9. 杨旭, 苗春妍, 谢彩侠, 李娟, 王丰青. 水杨酸对地黄毛蕊花糖苷含量及基因表达的影响. 植物科学学报, 2023, 41(2): 204-213.
10. 王丰青, 杨旭, 左鑫, 苗春妍, 张重义. 地黄全长转录组测序及苯乙醇苷合成途径催化酶基因鉴定. 药学学报, 2022, 57(3): 831-838.
11. 左鑫, 李铭铭, 李欣容, 苗春妍, 李炎枋, 杨旭, 张重义, 王丰青(通讯). CRISPR/Cas9 技术在天目地黄 RcPDS1 基因编辑中的应用. 园艺学报, 2022, 49(7): 1532-1544.
12. 谢彩侠, 李伟峰, 龚海燕, 李雅静, 张娟, 刘庆普, 雷敬卫, 王丰青(通讯). 低磷胁迫下盾叶薯蓣中甾体皂苷生物合成关键酶基因的筛选及鉴定. 中国中药杂志,2022, 47(10): 2623-2633.
13. 王丰青, 杨超飞, 李铭铭, 左鑫, 杨旭, 李欣容, 古力, 杜家方, 李明杰, 王浩, 张重义. 密度对地黄生长及基因转录特性的影响分析. 中国中药杂志, 2021, 46(17):439-451.
14. 李铭铭, 索艳飞, 左鑫, 李欣容, 谢彩侠, 王丰青(通讯). 地黄RgMYB10基因的克隆与表达分析. 西北植物学报, 2021, 41(2): 212-221.
15. 左鑫, 李欣容, 李铭铭, 杨超飞, 张重义, 王丰青(通讯). 基因编辑技术及其在药用植物中的应用展望. 中国现代中药, 2020, 22(12): 2108-2114.
16. 李欣容,智惊宇,杨超飞,李铭铭,左鑫,谢彩侠,王丰青(通讯). 地黄毛蕊花糖苷合酶基因的克隆、亚细胞定位与表达特性分析. 中草药, 2020, 51(18): 4739-4746.
17. 王丰青, 李欣容, 杨超飞, 智惊宇, 张雪丽, 古力, 谢彩侠, 张重义. 遮阴对地黄块根性状、光合特性及基因转录的影响. 中草药, 2019, 50(18): 4419-4429.
18. 杨超飞, 李欣容, 智惊宇, 耿晓桐, 洪利亚, 王丰青(通讯), 谢彩侠. 地黄环烯醚萜合酶基因的克隆、序列特征与表达分析. 中国中药杂志, 2019, 44(12): 2472-2479.
19. 王丰青, 谢彩侠, 孙瑞斌, 张重义. 地黄种质创新与品种选育研究进展. 中国中药杂志, 2018, 43(21): 4203-4209.
20. 李雅静, 王丰青(通讯), 谢彩侠, 智惊宇, 孟庆博, 耿晓桐, 陶晓赛, 张苗. 盾叶薯蓣转录组分析及其皂苷元生物合成关键酶基因的挖掘. 中草药, 2018, 49(16): 3885-3894.
21. 谢彩侠,李雅静,张苗,耿晓桐,王丰青(通讯),张重义. 不同种质地黄块根菊花心与非菊花心部位有效成分特征分析. 中国实验方剂学杂志, 2018, 24(20): 75-83.
22. 谢彩侠, 李雅静, 张苗, 耿晓桐, 雷敬卫, 王丰青(通讯), 张重义. 怀地黄中地黄苷A、地黄苷D及益母草苷含量快速分析方法的建立. 中国实验方剂学杂志, 2018, 24(6): 47-54.
23. 王丰青, 王丽娜, 智惊宇, 张苗, 杨超飞, 黄勇, 谢彩侠, 张重义. 不同品种地黄毛蕊花糖苷的动态积累规律变化. 中国实验方剂学杂志, 2017, 23(24): 78-83.
24. 谢彩侠, 张苗, 李雅静, 耿晓桐, 王丰青(通讯), 张重义. 怀地黄块根中菊花心与非菊花心部位的化学质量特征比较. 中国中药杂志, 2017, 42(21):4172-4179.
25. 王丽娜, 王丰青(通讯), 智惊宇, 张苗, 张重义, 谢彩侠. 盾叶薯蓣WRKY转录因子的鉴定和生物信息学分析, 中草药, 2016, 47(22): 4062-4071.
26. 王丰青, 杜家方, 李烜桢, 张雪丽, 高勇, 张宝堂, 张重义. 晚播对怀牛膝群体的调节效应研究. 中国现代中药, 2016, 18(9): 1167-1171.
27. 王丰青, 智惊宇, 谢彩侠, 杜家方, 索艳飞, 王海燕, 张重义. 地黄酪氨酸脱羧酶基因的克隆与表达分析. 中国中药杂志, 2016, 41(16):2981-2986.
28. 王丰青, 谢彩侠, 李明杰, 李烜桢, 张红瑞, 杜家方, 张重义. 地黄中介体亚基基因
RgMed6的分离与表达特性. 中草药, 2015, 46(19): 2919-2924.
29. 田云鹤, 王丰青(通讯), 李明杰, 索艳飞, 李连珍, 张宝, 张重义. 不同地黄种质农艺性状的连作障碍效应分析. 河南农业科学, 2015, 44(8): 98-103.
30. 王丰青, 田云鹤, 黄勇, 李娟, 董诚明, 张重义. 18个地黄种质的表型性状及相关统计学分析. 植物资源与环境学报, 2015, 24(1):28-35.
31. 王丰青, 周艳, 黄勇, 李明杰, 田云鹤, 冯法节, 陈新建, 张重义. 地黄扩展蛋白基因
RgEXPA10的克隆与表达分析. 药学学报, 2015, 50 (2): 233-240.
32. 王丰青, 田云鹤, 谢彩侠, 杜家方, 李烜桢, 张留记, 何华勤, 张重义. 根癌农杆菌介导的怀地黄遗传转化研究. 中草药, 2014, 45(17): 2541-2546.
33. 王丰青, 吴为人, 陈新建, 李娟, 周艳, 张重义. 串联亲和纯化技术及其在植物蛋白质组研究中的应用. 生命科学, 2013, 23(6): 632-638.
论著
1.《地黄连作障碍的形成机制》, 科学出版社, 2021.3, ISBN 978-7-03-068389-2, 参撰.
2.《药用植物育种学》, 高等教育出版社, 2020.12, ISBN 978-7-04-052763-6, 参编.
3.《药用植物栽培学》, 高等教育出版社, 2019.12, ISBN 978-7-04-055071-9, 参编.
4.《药用植物生理生态学》, 中国中医药出版社, 2012.8, ISBN 978-7-5132-1074-4, 参编.
5.《药用植物栽培学实验实习指导》, 高等教育出版社, 2020.8, ISBN 978-7-04-052824-4, 参编.
专利
1. 一种地黄WRKY转录因子
RgWRKY37基因及其应用. 专利号: ZL202110778447.1, 授权日: 2023.6.16, 排名第1.
2. 一种创制地黄杂合突变体的方法及应用. 专利号: ZL202110397674.X, 授权日: 2023.6.20, 排名第1.
3. 一种天目地黄花青素相关基因
RcMYB1及其应用. 专利号:ZL202111657700.4, 授权日: 2023.6.30, 排名第1.
4. 一条
RcMYB3基因及其在提高植物花青素含量中的应用. 专利号: ZL 202111657682.X, 授权日: 2023.6.30, 排名第1.
5. 一种利用地黄毛状根生产毛蕊花糖苷的方法. 专利号: ZL201610554590.1, 授权日期: 2019.5.17, 排名第1.
6. 一种miR1432靶基因串联模拟物、基因表达盒、表达载体、制备方法及应用. 专利号:ZL201310695733.7. 授权日期: 2016.1.20, 排名第5.
奖励与荣誉
1. 河南省优秀硕士学位论文指导教师(2022年和2023年)
2. 河南省优秀博士后研究人员(2015年)
3. 河南省教育厅科技成果奖优秀科技论文壹等奖(2018年)、贰等奖(2021年)
4. 河南农业大学优秀硕士学位论文指导教师(2021和2022年)
5. 河南农业大学本科毕业论文优秀指导教师(2016,2018,2021和2022年)
6. 河南农业大学教书育人先进个人(2018年)
7. 河南农业大学优秀教师(2017年)
8. 河南农业大学优秀班主任(2023年)
9. 河南农业大学大学生三下乡社会实践活动优秀指导教师(2014年)
10. 河南农业大学大学生实验创新计划项目优秀指导教师(2013年)